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Addgene

pUC118-mCtnnb1-FLIP-FlpE-Puro
(Plasmid #84541)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84541 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC118
  • Backbone manufacturer
    TAKARA
  • Vector type
    Mouse Targeting, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    suggested bacterial strain for expression is Stellar (HST08)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse beta-catenin FLIP-FlpE - Puro targeting vector
  • Species
    Synthetic
  • Insert Size (bp)
    3903

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC118-mCtnnb1-FLIP-FlpE-Puro was a gift from Bon-Kyoung Koo (Addgene plasmid # 84541 ; http://n2t.net/addgene:84541 ; RRID:Addgene_84541)

  • For your References section:

    One-step generation of conditional and reversible gene knockouts. Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156. 10.1038/nmeth.4156 PubMed 28135257
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