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Addgene

pAG415-GPDp-EGFP-rap1-K3NR
(Plasmid #84533)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG415
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RAP1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    K(43,117,118)R
  • Entrez Gene
    RAP1 (a.k.a. YNL216W, GRC4, GRF1, TBA1, TUF1)
  • Promoter GPD
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG415-GPDp-EGFP-rap1-K3NR was a gift from Jorrit Enserink (Addgene plasmid # 84533 ; http://n2t.net/addgene:84533 ; RRID:Addgene_84533)

  • For your References section:

    Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes. Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, Meza-Zepeda LA, Klungland A, Enserink JM. Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub 2015 Mar 23. 10.1101/gr.185793.114 PubMed 25800674
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