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Addgene

pDONR221-RAP1
(Plasmid #84525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Vector type
    Gateway donor plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RAP1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    no stop codon
  • Entrez Gene
    RAP1 (a.k.a. YNL216W, GRC4, GRF1, TBA1, TUF1)
  • Promoter none

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

No stop codon follows insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-RAP1 was a gift from Jorrit Enserink (Addgene plasmid # 84525 ; http://n2t.net/addgene:84525 ; RRID:Addgene_84525)

  • For your References section:

    Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes. Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, Meza-Zepeda LA, Klungland A, Enserink JM. Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub 2015 Mar 23. 10.1101/gr.185793.114 PubMed 25800674
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