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Addgene

pET30a-SRAP
(Plasmid #84524)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84524 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30a
  • Backbone manufacturer
    Millipore
  • Backbone size w/o insert (bp) 5422
  • Total vector size (bp) 6172
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRAP domain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    750
  • Entrez Gene
    Hmces (a.k.a. 8430410A17Rik, C85376)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • S-Tag (N terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a-SRAP was a gift from Douglas Feldman (Addgene plasmid # 84524 ; http://n2t.net/addgene:84524 ; RRID:Addgene_84524)

  • For your References section:

    Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease. Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE. Cell Rep. 2017 Oct 10;21(2):482-494. doi: 10.1016/j.celrep.2017.09.055. 10.1016/j.celrep.2017.09.055 PubMed 29020633
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