pEF HA-TSAdwt
(Plasmid
#84517)
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PurposeMammalian expression of TSAd
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84517 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF HA (pEF BOS derivative)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTSAd
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Alt nameSH2 domain containing 2A
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Alt nameSH2D2A
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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Entrez GeneSH2D2A (a.k.a. F2771, SCAP, TSAD, VRAP)
- Promoter EF1a
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EF1a-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF HA-TSAdwt was a gift from Anne Spurkland (Addgene plasmid # 84517 ; http://n2t.net/addgene:84517 ; RRID:Addgene_84517) -
For your References section:
Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7. Granum S, Sundvold-Gjerstad V, Dai KZ, Kolltveit KM, Hildebrand K, Huitfeldt HS, Lea T, Spurkland A. BMC Immunol. 2006 Jul 13;7:15. 10.1186/1471-2172-7-15 PubMed 16839418