pET29b-EPN01(∆L1+∆L2)-His
(Plasmid #84511)

• Purpose: E.coli version of EPN01(∆L1+∆L2)
• Depositing Lab: Neil King
• Publication: Votteler et al Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30.

Backbone

• Vector backbone: pET29b
• Backbone size w/o insert (bp): 5214
• Modifications to backbone: Inserts were cloned into the Nde1 and Xho1 sites
• Vector type: Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EPN01(∆L1+∆L2)-His
• Insert Size (bp): 873
• Tag / Fusion Protein:
  • His tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: GCT AGT TAT TGC TCA GCG G

Resource Information

• Supplemental Documents:
  • Myr1-12GS-I3-01-myc-Late1(deltaL)_pET29b.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry