V1-MESA-45F-Tev
(Plasmid #84501)

• Purpose: MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease
• Depositing Lab: Joshua Leonard
• Publication: Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5520
• Total vector size (bp): 7194
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: V1-MESA-45F-Tev
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1674
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Resource Information

• Supplemental Documents:
  • V1-MESA-45F-Tev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry