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Addgene

pLJC6-Flag CASTOR1 I280A
(Plasmid #84497)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84497 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pS2JC6
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CASTOR1 I280A
  • Species
    H. sapiens (human)
  • Mutation
    I280A
  • Entrez Gene
    GATSL3 (a.k.a. CASTOR1)
  • Promoter sestrin2
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer TAGGGTAGGCTCTCCTG
  • 3′ sequencing primer pLJM1R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJC6-Flag CASTOR1 I280A was a gift from David Sabatini (Addgene plasmid # 84497 ; http://n2t.net/addgene:84497 ; RRID:Addgene_84497)

  • For your References section:

    The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, Wang T, Harper JW, Gygi SP, Sabatini DM. Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. Epub 2016 Mar 10. 10.1016/j.cell.2016.02.035 PubMed 26972053
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