pRK5-Flag CASTOR1 I280A
(Plasmid
#84495)
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Purposeoverexpression of CASTOR1 I280A mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCASTOR1 I280A
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SpeciesH. sapiens (human)
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MutationI280A
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Entrez GeneGATSL3 (a.k.a. CASTOR1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer ATT TAG GTG ACA CTA TAG
- 3′ sequencing primer TGT AAC CAT TAT AAG CTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-Flag CASTOR1 I280A was a gift from David Sabatini (Addgene plasmid # 84495 ; http://n2t.net/addgene:84495 ; RRID:Addgene_84495) -
For your References section:
The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, Wang T, Harper JW, Gygi SP, Sabatini DM. Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. Epub 2016 Mar 10. 10.1016/j.cell.2016.02.035 PubMed 26972053