Skip to main content
Addgene

pRK5-HA CASTOR2 ACT2
(Plasmid #84494)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84494 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CASTOR2 ACT2
  • Species
    H. sapiens (human)
  • Mutation
    171-last amino acid
  • Entrez Gene
    GATSL2 (a.k.a. CASTOR2, GATSL1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer ATT TAG GTG ACA CTA TAG
  • 3′ sequencing primer TGT AAC CAT TAT AAG CTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-HA CASTOR2 ACT2 was a gift from David Sabatini (Addgene plasmid # 84494 ; http://n2t.net/addgene:84494 ; RRID:Addgene_84494)

  • For your References section:

    The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, Wang T, Harper JW, Gygi SP, Sabatini DM. Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. Epub 2016 Mar 10. 10.1016/j.cell.2016.02.035 PubMed 26972053
Related items:
Commonly requested with: