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Addgene

Mouse c-myc (exons II and III)
(Plasmid #8448)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mouse c-myc (exons II and III)
  • Alt name
    myc
  • Alt name
    c-myc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Mutation
    Exons II and III only
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Recloned from Kenneth Marcu's M54 cDNA described in Nature 1984 Aug 2-8;310(5976):423-5.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse c-myc (exons II and III) was a gift from Frederic Mushinski (Addgene plasmid # 8448 ; http://n2t.net/addgene:8448 ; RRID:Addgene_8448)

  • For your References section:

    Addition of constitutive c-myc expression to Abelson murine leukemia virus changes the phenotype of the cells transformed by the virus from pre-B-cell lymphomas to plasmacytomas. Weissinger EM, Mischak H, Goodnight J, Davidson WF, Mushinski JF. Mol Cell Biol 1993 Apr;13(4):2578-85. PubMed 8455630
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