Skip to main content
Addgene

pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
(Plasmid #84474)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Derived from AAV V032
  • Backbone manufacturer
    Rachael Neve
  • Total vector size (bp) 8368
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mKate2
  • Insert Size (bp)
    717
  • Promoter TRE
  • Tag / Fusion Protein
    • Nuclear localization signal of SV40 large T antigen (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age1 (not destroyed)
  • 3′ cloning site Asc1 (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    NLS-mKate2; from plasmid pAAV-EF1a-P2A-nls-mKate2 provided by Dr. Jonathan Ting (MIT, now at Allen Institute)
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more info regarding the plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA was a gift from Yingxi Lin (Addgene plasmid # 84474 ; http://n2t.net/addgene:84474 ; RRID:Addgene_84474)

  • For your References section:

    A robust activity marking system for exploring active neuronal ensembles. Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K, Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, Hemberg M, Yin JC, Maier SF, Lin Y. Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918. 10.7554/eLife.13918 PubMed 27661450
Related items:
Commonly requested with: