pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
(Plasmid #84474)

• Purpose: pAAV-RAM-NLS-mKate2
• Depositing Lab: Yingxi Lin
• Publication: Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.

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Backbone

• Vector backbone: Derived from AAV V032
• Backbone manufacturer: Rachael Neve
• Total vector size (bp): 8368
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mKate2
• Insert Size (bp): 717
• Promoter: TRE
• Tag / Fusion Protein:
  • Nuclear localization signal of SV40 large T antigen (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Age1 (not destroyed)
• 3′ cloning site: Asc1 (not destroyed)
• 5′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: NLS-mKate2; from plasmid pAAV-EF1a-P2A-nls-mKate2 provided by Dr. Jonathan Ting (MIT, now at Allen Institute)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more info regarding the plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918