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Addgene

pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA
(Plasmid #84468)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Derived from AAV V032
  • Backbone manufacturer
    Rachael Neve
  • Total vector size (bp) 9347
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Insert Size (bp)
    1431
  • Mutation
    T230I (please see depositor comments below)
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    tdTomato; from plasmid pAAV-hSyn-iCre-dTomato (JT) provided by Dr. Jonathan Ting (MIT, now at Allen Institute)
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation T230I in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.

For more info regarding the plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA was a gift from Yingxi Lin (Addgene plasmid # 84468 ; http://n2t.net/addgene:84468 ; RRID:Addgene_84468)

  • For your References section:

    A robust activity marking system for exploring active neuronal ensembles. Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K, Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, Hemberg M, Yin JC, Maier SF, Lin Y. Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918. 10.7554/eLife.13918 PubMed 27661450
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