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Addgene

pRN111
(Plasmid #84463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJB38-NWMN2930
  • Backbone size w/o insert (bp) 8815
  • Total vector size (bp) 9939
  • Selectable markers
    also contains Chloramphenicol resistance gene, but only functional in Gram-postive bacteria.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E.coli DC10b (described in doi: 10.1128/mBio.00537-12)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SarA_P1-mCherry from pRN11
  • Insert Size (bp)
    1124

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer CATAATGTGTTGTAAACATTTTTTTTG
  • 3′ sequencing primer TATGTCACTTATCCTTTTGGAAATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRN111 was a gift from Reindert Nijland (Addgene plasmid # 84463 ; http://n2t.net/addgene:84463 ; RRID:Addgene_84463)

  • For your References section:

    Fluorescent reporters for markerless genomic integration in Staphylococcus aureus. de Jong NW, van der Horst T, van Strijp JA, Nijland R. Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889. 10.1038/srep43889 PubMed 28266573
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