pTH100
(Plasmid #84458)

• Purpose: pJB38-NWMN29-30 + SarA_P1-sGFP-Term
• Depositing Lab: Reindert Nijland
• Publication: de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.

Backbone

• Vector backbone: pJB38-NWMN2930
• Backbone size w/o insert (bp): 8815
• Total vector size (bp): 9969
• Selectable markers: also contains Chloramphenicol resistance gene, but only functional in Gram-postive bacteria.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E.coli DC10b (described in doi: 10.1128/mBio.00537-12)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SarA_P1-sGFP from pCM29
• Insert Size (bp): 1154
• Tag / Fusion Protein:
  • 6xHis (on sfGFP) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: CATAATGTGTTGTAAACATTTTTTTTG
• 3′ sequencing primer: TATGTCACTTATCCTTTTGGAAATG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry