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Addgene

pSF4 TET CMV intron renilla 24xPP7 24xMS2 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 84444 with ClaI and EcoRI-HF.

We predict the following banding patterns:

ClaI single digest: 10.7kb

EcoRI-HF single digest: 10.7kb

ClaI / EcoRI-HF double digest: 6.2kb & 4.5kb

We see the expected result.