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Addgene

pSF4 CMV intron renilla TRICK CTE polyA Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 84443 with ClaI and EcoRI-HF.

We predict the following banding patterns:

ClaI single digest: 9.8kb

EcoRI-HF single digest: 9.8kb

ClaI / EcoRI-HF double digest: 6.2kb & 3.6kb

We see the expected result.