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Addgene

pUC19 PKC epsilon
(Plasmid #8442)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mouse Protein Kinase C epsilon
  • Alt name
    PKC epsilon
  • Alt name
    PKCe
  • Alt name
    Protein Kinase C epsilon
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Entrez Gene
    Prkce (a.k.a. 5830406C15Rik, PKC[e], PKCepsilon, Pkce)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 Forward20
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19 PKC epsilon was a gift from Frederic Mushinski (Addgene plasmid # 8442 ; http://n2t.net/addgene:8442 ; RRID:Addgene_8442)

  • For your References section:

    Overexpression of protein kinase C-delta and -epsilon in NIH 3T3 cells induces opposite effects on growth, morphology, anchorage dependence, and tumorigenicity. Mischak H, Goodnight JA, Kolch W, Martiny-Baron G, Schaechtle C, Kazanietz MG, Blumberg PM, Pierce JH, Mushinski JF. J Biol Chem 1993 Mar 25;268(9):6090-6. PubMed 8454583
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