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Addgene

pAd-Track SIRT1 H355A
(Plasmid #8441)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8441 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAd-Track-CMV
  • Backbone size w/o insert (bp) 9220
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SIRT1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Mutation
    Histidine 355 mutated to Alanine
  • Entrez Gene
    Sirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer No
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-Track SIRT1 H355A was a gift from Pere Puigserver (Addgene plasmid # 8441 ; http://n2t.net/addgene:8441 ; RRID:Addgene_8441)

  • For your References section:

    Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P. Nature 2005 Mar 3;434(7029):113-8. 10.1038/nature03354 PubMed 15744310
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