TOPO-BC2
(Plasmid
#84396)
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PurposeEncodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84396 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerThermo Fisher Scientific
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Vector typeBarcoding plasmids
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBC2
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SpeciesSynthetic
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Insert Size (bp)86
- Promoter SP6/T7
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is part of the FREQ-Seq-Squared kit. See Addgene's kit collection page for more details: https://www.addgene.org/kits/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TOPO-BC2 was a gift from J.J. Emerson (Addgene plasmid # 84396 ; http://n2t.net/addgene:84396 ; RRID:Addgene_84396) -
For your References section:
FREQ-Seq2: a method for precise high-throughput combinatorial quantification of allele frequencies. Zhao R, Lukacsovich T, Gaut R, Emerson JJ. G3 (Bethesda). 2023 Sep 30;13(10):jkad162. doi: 10.1093/g3journal/jkad162. 10.1093/g3journal/jkad162 PubMed 37494033
