PPRE-H2B-eGFP
(Plasmid #84393)

• Purpose: Fluorescent reporter for PPARgamma activity
• Depositing Labs: Severine Degrelle, Thierry Fournier
• Publication: Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1.

Backbone

• Vector backbone: H2B-GFP
• Backbone size w/o insert (bp): 5100
• Total vector size (bp): 6000
• Modifications to backbone: CMV promotor was removed and replaced by PPRE sequence
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPARG response element
• Alt name: PPRE
• Alt name: ApoCIII promotor
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Entrez Gene: PPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
• Promoter: PPRE
• Tag / Fusion Protein:
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AseI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: PPRE-AseI
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Geoff Wahl (Addgene plasmid # 11680)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry