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Addgene

pENTR CMVie-Lifeact-iRFP670 L1-R5
(Plasmid #84390)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84390 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMuLE ENTR MCS L1-R5
  • Backbone size w/o insert (bp) 3028
  • Total vector size (bp) 4031
  • Vector type
    Mammalian Expression ; Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lifeact
  • Species
    Synthetic
  • Insert Size (bp)
    51
  • Promoter CMV immediate early
  • Tag / Fusion Protein
    • iRFP670 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer M13-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Vladislav Verkhusha, Addgene #45457

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This truncated CMV promoter is not silenced in neurons.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR CMVie-Lifeact-iRFP670 L1-R5 was a gift from Ghassan Mouneimne (Addgene plasmid # 84390 ; http://n2t.net/addgene:84390 ; RRID:Addgene_84390)

  • For your References section:

    The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Padilla-Rodriguez M, Parker SS, Adams DG, Westerling T, Puleo JI, Watson AW, Hill SM, Noon M, Gaudin R, Aaron J, Tong D, Roe DJ, Knudsen B, Mouneimne G. Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2. 10.1038/s41467-018-05367-2 [pii] PubMed 30061623
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