Skip to main content
Addgene

pENTR CMVie-Lifeact-mRuby2 L1-R5
(Plasmid #84389)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMuLE ENTR MCS L1-R5
  • Backbone size w/o insert (bp) 3028
  • Total vector size (bp) 3819
  • Vector type
    Mammalian Expression ; Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lifeact
  • Species
    Synthetic
  • Insert Size (bp)
    51
  • Promoter CMV immediate early
  • Tag / Fusion Protein
    • mRuby2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer M13-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Michael Davidson, Addgene #54674. Sebastian Zeltzer, University of Arizona.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This truncated CMV promoter is not silenced in neurons.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR CMVie-Lifeact-mRuby2 L1-R5 was a gift from Ghassan Mouneimne (Addgene plasmid # 84389 ; http://n2t.net/addgene:84389 ; RRID:Addgene_84389)

  • For your References section:

    High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons. Parker SS, Moutal A, Cai S, Chandrasekaran S, Roman MR, Koshy AA, Khanna R, Zinsmaier KE, Mouneimne G. eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-0135-18. doi: 10.1523/ENEURO.0135-18.2018. eCollection 2018 Sep-Oct. eN-MNT-0135-18 [pii] PubMed 30263951
Related items: