pLenti Lifeact-mRuby2 BlastR
(Plasmid #84384)

• Purpose: Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons
• Depositing Lab: Ghassan Mouneimne
• Publication: Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-0135-18. doi: 10.1523/ENEURO.0135-18.2018. eCollection 2018 Sep-Oct.

Backbone

• Vector backbone: pMuLE Dest BlastR
• Backbone size w/o insert (bp): 3834
• Total vector size (bp): 8511
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Lifeact
• Species: Synthetic
• Insert Size (bp): 51
• Promoter: CMV immediate early
• Tag / Fusion Protein:
  • mRuby2 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV_fwd_primer
• 3′ sequencing primer: MSCV_rev_primer

Resource Information

• Supplemental Documents:
  • Datasheet for pLenti Lifeact-mRuby2 BlastR
• A portion of this plasmid was derived from a plasmid made by: Michael Davidson, Addgene #54674. Sebastian Zeltzer, University of Arizona.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry