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Addgene

pLenti Lifeact-EGFP BlastR
(Plasmid #84383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMuLE Dest BlastR
  • Backbone size w/o insert (bp) 3834
  • Total vector size (bp) 8518
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Lifeact
  • Species
    Synthetic
  • Insert Size (bp)
    51
  • Promoter CMV immediate early
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer MSCV_rev_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti Lifeact-EGFP BlastR was a gift from Ghassan Mouneimne (Addgene plasmid # 84383 ; http://n2t.net/addgene:84383 ; RRID:Addgene_84383)

  • For your References section:

    The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Padilla-Rodriguez M, Parker SS, Adams DG, Westerling T, Puleo JI, Watson AW, Hill SM, Noon M, Gaudin R, Aaron J, Tong D, Roe DJ, Knudsen B, Mouneimne G. Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2. 10.1038/s41467-018-05367-2 [pii] PubMed 30061623
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