pRH2521
(Plasmid #84380)

• Purpose: Expression of sgRNA from a imyc promoter (Pimyc)
• Depositing Lab: Robert Husson
• Publication: Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw625.

Backbone

• Vector backbone: pTE-10M-0X
• Backbone manufacturer: Addgene 20318
• Backbone size w/o insert (bp): 6608
• Total vector size (bp): 6638
• Modifications to backbone: The sequence Pmyc1tetO (2) followed by 2 BbsI sites and the sgRNA sequences were synthesized and cloned in pTE-10M-0X at a ClaI site to produce pRH2521
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sgRNA cloning site
• Insert Size (bp): 30

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry