Skip to main content
Addgene

pIRES-hSphK2-EGFP
(Plasmid #84371)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 7300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPHK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Entrez Gene
    SPHK2 (a.k.a. SK 2, SK-2, SPK 2, SPK-2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-hSphK2-EGFP was a gift from Anthony Don & Hugh Rosen (Addgene plasmid # 84371 ; http://n2t.net/addgene:84371 ; RRID:Addgene_84371)

  • For your References section:

    Essential requirement for sphingosine kinase 2 in a sphingolipid apoptosis pathway activated by FTY720 analogues. Don AS, Martinez-Lamenca C, Webb WR, Proia RL, Roberts E, Rosen H. J Biol Chem. 2007 May 25;282(21):15833-42. Epub 2007 Mar 30. 10.1074/jbc.M609124200 PubMed 17400555
Related items:
Commonly requested with: