pAcGFP-C2-hSphK2
(Plasmid #84370)

• Purpose: Expression of AcGFP-SphK2 fusion protein in mammalian cells
• Depositing Labs: Anthony Don, Hugh Rosen
• Publication: Don et al Biochem Biophys Res Commun. 2009 Feb 27;380(1):87-92. doi: 10.1016/j.bbrc.2009.01.075. Epub 2009 Jan 23.

Backbone

• Vector backbone: pAcGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4735
• Total vector size (bp): 6735
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SPHK2
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: SPHK2 (a.k.a. SK 2, SK-2, SPK 2, SPK-2)
• Promoter: CMV
• Tag / Fusion Protein:
  • AcGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: AcGFP1-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry