hP14Gal4
(Plasmid #84364)

• Purpose: Gal4-PRDM14 funsion plasmid for luciferase recruitment assay
• Depositing Lab: Danny Reinberg
• Publication: Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi: 10.1038/nature18004.

Backbone

• Vector backbone: pcDNA4/TO
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 5078
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRDM14
• Species: H. sapiens (human)
• Mutation: silent mutations at coding silent 1068CtoT, 1629T to C
• Entrez Gene: PRDM14 (a.k.a. PFM11)
• Promoter: CMV/TO
• Tags / Fusion Proteins:
  • HA tag (C terminal on insert)
  • Gal4 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry