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Addgene

CAGhP14
(Plasmid #84362)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84362 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Backbone manufacturer
    Dr. Peter Andrews
  • Backbone size w/o insert (bp) 7201
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRDM14
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1767
  • Mutation
    silent mutations at coding silent 1068CtoT, 1629T to C
  • Entrez Gene
    PRDM14 (a.k.a. PFM11)
  • Promoter CAG
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAGhP14 was a gift from Danny Reinberg (Addgene plasmid # 84362 ; http://n2t.net/addgene:84362 ; RRID:Addgene_84362)

  • For your References section:

    Co-repressor CBFA2T2 regulates pluripotency and germline development. Tu S, Narendra V, Yamaji M, Vidal SE, Rojas LA, Wang X, Kim SY, Garcia BA, Tuschl T, Stadtfeld M, Reinberg D. Nature. 2016 Jun 8;534(7607):387-90. doi: 10.1038/nature18004. 10.1038/nature18004 PubMed 27281218
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