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Addgene

pMal-MATa1
(Plasmid #8436)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pMal-c2x
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MATa1(78aa-126aa)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    144
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site pstI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pMALE
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMal-MATa1 was a gift from Cynthia Wolberger (Addgene plasmid # 8436 ; http://n2t.net/addgene:8436 ; RRID:Addgene_8436)

  • For your References section:

    Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding protein chimera. Ke A, Wolberger C. Protein Sci 2003 Feb;12(2):306-12. 10.1110/ps.0219103 PubMed 12538894
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