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Addgene

mVenus-C1-GEFT
(Plasmid #84340)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84340 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmVenus-C1
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6111
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GEFT
  • Alt name
    p63RhoGEF (474 aa)
  • Alt name
    ARHGEF25
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1422
  • Entrez Gene
    ARHGEF25 (a.k.a. GEFT, p63RhoGEF)
  • Promoter CMV
  • Tag / Fusion Protein
    • mVenus (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mVenus-C1-GEFT was a gift from Dorus Gadella (Addgene plasmid # 84340 ; http://n2t.net/addgene:84340 ; RRID:Addgene_84340)

  • For your References section:

    Kinetics of recruitment and allosteric activation of ARHGEF25 isoforms by the heterotrimeric G-protein Galphaq. van Unen J, Yin T, Wu YI, Mastop M, Gadella TW Jr, Goedhart J. Sci Rep. 2016 Nov 11;6:36825. doi: 10.1038/srep36825. 10.1038/srep36825 PubMed 27833100
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