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Addgene

pLck-mVenus-C1
(Plasmid #84337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmVenus-C1
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 4764
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lck (MGCVCSSNPELPVAT)
  • Alt name
    Plasma membrane targeting sequence
  • Species
    Synthetic
  • Insert Size (bp)
    45
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLck-mVenus-C1 was a gift from Dorus Gadella (Addgene plasmid # 84337 ; http://n2t.net/addgene:84337 ; RRID:Addgene_84337)

  • For your References section:

    The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling. van Unen J, Botman D, Yin T, Wu YI, Hink MA, Gadella TWJ Jr, Postma M, Goedhart J. BMC Cell Biol. 2018 Jun 7;19(1):6. doi: 10.1186/s12860-018-0155-2. 10.1186/s12860-018-0155-2 PubMed 29879899
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