mCherry-C1-TGAT
(Plasmid #84334)

• Purpose: Expresses TGAT tagged with mCherry
• Depositing Lab: Dorus Gadella
• Publication: van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi: 10.1186/s12860-018-0155-2.

Backbone

• Vector backbone: pmCherry-C1
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5490
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGAT
• Alt name: splice variant of the RhoGEF Trio with mutations
• Species: H. sapiens (human)
• Insert Size (bp): 765
• Mutation: WT
• Entrez Gene: TRIO (a.k.a. ARHGEF23, MEBAS, MRD44, MRD63, tgat)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry