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PurposeCo-expresses (untagged) Histamine-1 receptor and mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 84330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 6230
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH1R-P2A-mCherry
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Alt nameHistamine 1 receptor
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SpeciesH. sapiens (human)
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Entrez GeneHRH1 (a.k.a. H1-R, H1R, HH1R, hisH1)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH1R-P2A-mCherry-N1 was a gift from Dorus Gadella (Addgene plasmid # 84330 ; http://n2t.net/addgene:84330 ; RRID:Addgene_84330) -
For your References section:
Quantitative Single-Cell Analysis of Signaling Pathways Activated Immediately Downstream of Histamine Receptor Subtypes. van Unen J, Rashidfarrokhi A, Hoogendoorn E, Postma M, Gadella TW Jr, Goedhart J. Mol Pharmacol. 2016 Sep;90(3):162-76. doi: 10.1124/mol.116.104505. Epub 2016 Jun 29. 10.1124/mol.116.104505 PubMed 27358232