pP2A-mCherry-N1
(Plasmid #84329)

• Purpose: Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry
• Depositing Lab: Dorus Gadella
• Publication: van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. doi: 10.1124/mol.116.104505. Epub 2016 Jun 29.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 4790
• Modifications to backbone: Inserted P2A sequence between MCS and mCherry
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: P2A-mCherry
• Alt name: 2A "self-cleaving" peptide
• Species: Synthetic
• Insert Size (bp): 90
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry