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Addgene

pH2R-mCherry-N1
(Plasmid #84326)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84326 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5801
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2R
  • Alt name
    Histamine 2 receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1818
  • Entrez Gene
    HRH2 (a.k.a. H2R, HH2R)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH2R-mCherry-N1 was a gift from Dorus Gadella (Addgene plasmid # 84326 ; http://n2t.net/addgene:84326 ; RRID:Addgene_84326)

  • For your References section:

    Quantitative Single-Cell Analysis of Signaling Pathways Activated Immediately Downstream of Histamine Receptor Subtypes. van Unen J, Rashidfarrokhi A, Hoogendoorn E, Postma M, Gadella TW Jr, Goedhart J. Mol Pharmacol. 2016 Sep;90(3):162-76. doi: 10.1124/mol.116.104505. Epub 2016 Jun 29. 10.1124/mol.116.104505 PubMed 27358232
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