pISH-Pou2f2
(Plasmid
#84320)
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PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f2. Not for gene expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePou2f2
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SpeciesM. musculus (mouse)
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Insert Size (bp)502
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Entrez GenePou2f2 (a.k.a. Oct-2, Oct2a, Oct2b, Otf-2, Otf2)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NcoI digestion and SP6 RNA polymerase for antisense probe
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Pou2f2 was a gift from Peter Mombaerts (Addgene plasmid # 84320 ; http://n2t.net/addgene:84320 ; RRID:Addgene_84320) -
For your References section:
Expression of homeobox genes in the mouse olfactory epithelium. Parrilla M, Chang I, Degl'Innocenti A, Omura M. J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. Epub 2016 Jun 19. 10.1002/cne.24051 PubMed 27243442
