pcDNA5/FRT/TO/SH/GW-Mst1
(Plasmid #84299)

• Purpose: Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.
• Depositing Lab: Jonathan Chernoff
• Publication: Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71. doi: 10.1074/jbc.M112.414524. Epub 2013 Feb 5.

Backbone

• Vector backbone: pcDNA5/FRT/TO/SH/GW
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mst1
• Alt name: Stk4
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Entrez Gene: STK4 (a.k.a. KRS2, MST1, YSK3)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA, SBP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene that can be regulated by tetracycline.