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Addgene

pUC19 PKC alpha
(Plasmid #8429)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8429 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mouse Protein Kinase C alpha
  • Alt name
    Protein Kinase C alpha
  • Alt name
    PKC alpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • GenBank ID
    M25811
  • Entrez Gene
    Prkca (a.k.a. Pkca)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 Forward20
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Stefan Rose-John

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please cite "Gene. 1988 Dec 30;74(2):465-71" if you use this plasmid in your publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19 PKC alpha was a gift from Frederic Mushinski (Addgene plasmid # 8429 ; http://n2t.net/addgene:8429 ; RRID:Addgene_8429)
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