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Addgene

pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA
(Plasmid #84274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Plenti-7.3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3475
  • Total vector size (bp) 11161
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flag-GluN2A
  • Insert Size (bp)
    7686
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu1 (unknown if destroyed)
  • 3′ cloning site Blp1 (unknown if destroyed)
  • 5′ sequencing primer tagtagacataatagcaacag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA was a gift from Jonathan Ploski (Addgene plasmid # 84274 ; http://n2t.net/addgene:84274 ; RRID:Addgene_84274)

  • For your References section:

    The production of viral vectors designed to express large and difficult to express transgenes within neurons. Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE. Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7. 10.1186/s13041-015-0100-7 PubMed 25887710
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