Skip to main content
Addgene

pMyCA-mCherry
(Plasmid #84272)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMyCA
  • Backbone size w/o insert (bp) 5994
  • Total vector size (bp) 6684
  • Vector type
    Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Expression using M.smegmatis
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Discosoma sp
  • Insert Size (bp)
    708
  • Promoter Acetamidase
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer cgagaacctgtacttccagggcatggtgagcaagggcgag
  • 3′ sequencing primer gcctggcagtcgatcgtacgttacttgtacagctcgtcc
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMyCA-mCherry was a gift from Young-Hwa Song (Addgene plasmid # 84272 ; http://n2t.net/addgene:84272 ; RRID:Addgene_84272)

  • For your References section:

    A versatile vector for mycobacterial protein production with a functional minimized acetamidase regulon. Magana Vergara C, Kallenberg CJL, Rogasch M, Hubner CG, Song YH. Protein Sci. 2017 Aug 31. doi: 10.1002/pro.3288. 10.1002/pro.3288 PubMed 28857325
Related items:
Commonly requested with: