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Addgene

pUC19 PKC delta
(Plasmid #8427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mouse Protein Kinase C delta
  • Alt name
    PKC delta
  • Alt name
    PKCd
  • Alt name
    Protein Kinase C delta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2600
  • Entrez Gene
    Prkcd (a.k.a. D14Ertd420e, PKC[d], PKCdelta, Pkcd)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 Forward20
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19 PKC delta was a gift from Frederic Mushinski (Addgene plasmid # 8427 ; http://n2t.net/addgene:8427 ; RRID:Addgene_8427)

  • For your References section:

    Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein. Mischak H, Bodenteich A, Kolch W, Goodnight J, Hofer F, Mushinski JF. Biochemistry 1991 Aug 13;30(32):7925-31. 10.1021/bi00246a008 PubMed 1868068
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