pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP
(Plasmid #84255)

• Purpose: Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker
• Depositing Lab: Stanley Qi
• Publication: Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.

Backbone

• Vector backbone: pHR
• Total vector size (bp): 7646
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sa sgCXCR4-2
• gRNA/shRNA sequence: GCCCAGGGGACCCTGCTGTTT
• Species: H. sapiens (human), Synthetic; S. aureus
• Promoter: mouse U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstXI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GAGATCCAGTTTGGTTAGTACCGGG
• 3′ sequencing primer: ATGCATGGCGGTAATACGGTTAT

Resource Information

• Supplemental Documents:
  • pSLQ2853-4 Annotated GenBank File
• A portion of this plasmid was derived from a plasmid made by: The original Sa sgRNA scaffold was a gift from Feng Zhang (MIT) and was modified based on a design by Bo Huang (UCSF).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains a CMV-EGFP fluorescent marker 3' of the sgRNA.