pUC19 PKC zeta
(Plasmid
#8425)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8425 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Backbone manufacturerATCC
- Backbone size w/o insert (bp) 2700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse Protein Kinase C zeta
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Alt namePKC zeta
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Alt namePKCz
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Alt nameProtein Kinase C zeta
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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GenBank IDM94632
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Entrez GenePrkcz (a.k.a. Pkcz, aPKCzeta, nPKC-zeta, zetaPKC)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HincII (unknown if destroyed)
- 3′ cloning site HincII (unknown if destroyed)
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer M13 Forward20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19 PKC zeta was a gift from Frederic Mushinski (Addgene plasmid # 8425 ; http://n2t.net/addgene:8425 ; RRID:Addgene_8425) -
For your References section:
The cDNA sequence, expression pattern and protein characteristics of mouse protein kinase C-zeta. Goodnight J, Kazanietz MG, Blumberg PM, Mushinski JF, Mischak H. Gene 1992 Dec 15;122(2):305-11. 10.1016/0378-1119(92)90219-F PubMed 1487145