iCas Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid 84232 with NotI-HF and XbaI.
We predict the following banding patterns:
NotI-HF single digest: 13kb
XbaI single digest: 13kb
NotI-HF / XbaI double digest: 8kb & 5kb
We see the expected result.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid iCas with the following enzymes: NotI-HF and PmeI.
Expected Bands
NotI-HF/ PmeI: 8.8 kb & 4.2 kb
We observe the expected bands for this digest
