pMK1200
(Plasmid
#84219)
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Purpose(Empty Backbone) Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSicoR
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK1200 was a gift from Martin Kampmann & Jonathan Weissman (Addgene plasmid # 84219 ; http://n2t.net/addgene:84219 ; RRID:Addgene_84219) -
For your References section:
Integrated platform for genome-wide screening and construction of high-density genetic interaction maps in mammalian cells. Kampmann M, Bassik MC, Weissman JS. Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):E2317-26. doi: 10.1073/pnas.1307002110. Epub 2013 Jun 5. 10.1073/pnas.1307002110 PubMed 23739767
Map uploaded by the depositor.
