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Addgene

pAcUW21-CqJHE
(Plasmid #84213)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcUW21
  • Backbone manufacturer
    BD
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    JHE
  • Species
    Culex quinquefasciatus
  • Promoter p10

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer p2Bac-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcUW21-CqJHE was a gift from Bruce Hammock (Addgene plasmid # 84213 ; http://n2t.net/addgene:84213 ; RRID:Addgene_84213)

  • For your References section:

    Juvenile hormone (JH) esterase of the mosquito Culex quinquefasciatus is not a target of the JH analog insecticide methoprene. Kamita SG, Samra AI, Liu JY, Cornel AJ, Hammock BD. PLoS One. 2011;6(12):e28392. doi: 10.1371/journal.pone.0028392. Epub 2011 Dec 9. 10.1371/journal.pone.0028392 PubMed 22174797
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