pAcUW21-hvmeh1
(Plasmid
#84212)
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Purposebaculovirus transfer vector containing hvmeh1 coding sequence from Heliothis virescent
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84212 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAcUW21
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Backbone manufacturerBD
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemeh1
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Alt namemicrosomal epoxide hydrolase
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SpeciesHeliothis virescens
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GenBank IDJX681816
- Promoter p10
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer p2Bac-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcUW21-hvmeh1 was a gift from Bruce Hammock (Addgene plasmid # 84212 ; http://n2t.net/addgene:84212 ; RRID:Addgene_84212) -
For your References section:
Cloning and characterization of a microsomal epoxide hydrolase from Heliothis virescens. Kamita SG, Yamamoto K, Dadala MM, Pha K, Morisseau C, Escaich A, Hammock BD. Insect Biochem Mol Biol. 2013 Mar;43(3):219-28. doi: 10.1016/j.ibmb.2012.12.002. Epub 2012 Dec 28. 10.1016/j.ibmb.2012.12.002 PubMed 23276675