pLTR PKC zeta
(Plasmid
#8421)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLTR
- Backbone size w/o insert (bp) 7835
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Vector typeMammalian Expression
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Selectable markersxanthine-guanine phosphophorbosyltransferase (Mulligan and Berg, 1981)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse Protein Kinase C zeta
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Alt namePKC zeta
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Alt namePKCz
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Alt nameProtein Kinase C zeta
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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Entrez GenePrkcz (a.k.a. Pkcz, aPKCzeta, nPKC-zeta, zetaPKC)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypLTR was a gift from Dr. Nachman Mazurek (map of empty pLTR backbone uploaded here).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLTR PKC zeta was a gift from Frederic Mushinski (Addgene plasmid # 8421 ; http://n2t.net/addgene:8421 ; RRID:Addgene_8421) -
For your References section:
Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta. Mischak H, Pierce JH, Goodnight J, Kazanietz MG, Blumberg PM, Mushinski JF. J Biol Chem 1993 Sep 25;268(27):20110-5. PubMed 8376369
Map uploaded by the depositor.